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eBook Flow Cytometry Protocols (Methods in Molecular Biology) ePub

by Teresa S. Hawley,Robert Hawley

eBook Flow Cytometry Protocols (Methods in Molecular Biology) ePub
Author: Teresa S. Hawley,Robert Hawley
Language: English
ISBN: 1588292355
ISBN13: 978-1588292353
Publisher: Humana Press; 2nd ed. 2004 edition (March 18, 2004)
Pages: 434
Category: Medicine
Subcategory: Medical
Rating: 4.2
Votes: 246
Formats: docx doc lrf rtf
ePub file: 1494 kb
Fb2 file: 1650 kb

Imaging Flow Cytometry: Methods and Protocols (Methods in Molecular Biology)

Imaging Flow Cytometry: Methods and Protocols (Methods in Molecular Biology). Natasha S. Barteneva. Series: Methods in Molecular Biology (Book 1678).

Flow Cytometry Protocols. Includes cutting-edge methods and protocols. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Show all. About the authors. Paves the way for flow cytometry's use in the study of systems biology. Includes extensive expert notes to ensure optimal results. As a volume in the highly successful Methods in Molecular Biology™ series, chapters contain brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and extensive notes on troubleshooting and avoiding known pitfalls.

Flow Cytometry Protocols

Flow Cytometry Protocols. The protocols presented follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Book · January 2011 with 8 Reads.

Flow Cytometry Protocols (Methods in Molecular Biology), 2nd ed. Teresa S. Hawley, Robert G. Hawley. Download (pdf, . 0 Mb).

Flow Cytometry Protocols book . As a volume in the highly successful Methods in Molecular Biology series, chapters contain brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and extensive notes on troubleshooting and avoiding known pitfalls.

As a volume in the highly successful Methods in Molecular Biology™ series, chapters contain brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and extensive notes on troubleshooting and avoiding known pitfalls.

William G. Telford, Teresa S. Hawley, +2 authors Robert G. Published in Methods 2012.

355. 356 Strack, Keenan, and Glick. Like the parental DsRed-Express2, these new variants substantially outperform other FPs with regard to cytotoxicity in bacterial and mammalian systems (17–19). E2-Orange is ideal for multi-color experiments involv-ing green and far-red FPs (Fig. 1a), while E2-Red/Green is use-ful as a third color for ow cytometry in combination with red and green FPs (Fig. 1b).

Flow cytometry has evolved since the 1940s into a multidisciplinary field incorporating aspects of laser technology, fluid dynamics, electronics, optics, computer science, physics, chemistry, biology, and mathematics. Innovations in instrumentation, development of small lasers, discovery of new fluorochromes/fluorescent proteins, and implementation of novel methodologies have all contributed to the recent rapid expansion of flow cytometry applications. In this thoroughly revised and updated second edition of Flow Cytometry Protocols, time-proven as well as cutting-edge methods are clearly and comprehensively presented by leading experimentalists. In addition to being a valuable reference manual for experienced flow cytometrists, the editors expect this authoritative up-to-date collection to prove useful to investigators in all areas of the biological and biomedical sciences who are new to the subject. The introductory chapter provides an eloquent synopsis of the principles and diverse uses of flow cytometry, beginning with a historical perspective and ending with a view to the future. Chapters 2–22 contain step-by-step protocols of highly practical and state-of-the-art techniques. Detailed instructions and helpful tips on experimental design, as well as selection of reagents and data analysis tools, will allow researchers to readily carry out flow cytometric investigations ranging from traditional phenotypic characterizations to emerging genomics and proteomics applications. Complementing these instructive protocols is a chapter that provides a preview of the next generation of solid-state lasers, and one that describes a rapid means to validate containment of infectious aerosols generated during high-speed sorting (Chapters 23–24).
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